Please use this page to submit a request for cell lines listed below.
Cell line #1: WTC11-dCas9
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iPSC line with stable expression of dCas9-KRAB (CLYBL locus knock-in of CAG-dCas9-KRAB)
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Yang X, Wen J, Yang H, Jones IR, Zhu X, Liu W, Li B, Clelland CD, Luo W, Wong MY, Ren X, Cui X, Song M, Liu H, Chen C, Eng N, Ravichandran M, Sun Y, Lee D, Van Buren E, Jiang MZ, Chan CSY, Ye CJ, Perera RM, Gan L, Li Y, Shen Y. Functional characterization of Alzheimer’s disease genetic variants in microglia. Nature Genetics. 2023 Sep 21. doi: 10.1038/s41588-023-01506-8. PMID: 37735198
Cell line #2: WTC11-iNgn2-dCas9
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iPSC line with stable expression of dCas9-KRAB and doxycycline inducible expression of Ngn2 (CLYBL locus knock-in of CAG-dCas9-KRAB)
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Yang X, Jones IR, Chen PB, Yang H, Ren X, Zheng L, Li B, Li YE, Sun Q, Wen J, Beaman C, Cui X, Li Y, Wang W, Hu M, Ren B, Shen Y. Functional characterization of gene regulatory elements and neuropsychiatric disease-associated risk loci in iPSCs and iPSC-derived neurons. bioRxiv. 2023 Aug 30. doi: 10.1101/2023.08.30.555359.
Cell line #3: H1-dCas9
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hESC line with stable expression of dCas9-KRAB (CLYBL locus knock-in of CAG-dCas9-KRAB)
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Yang X, Wen J, Yang H, Jones IR, Zhu X, Liu W, Li B, Clelland CD, Luo W, Wong MY, Ren X, Cui X, Song M, Liu H, Chen C, Eng N, Ravichandran M, Sun Y, Lee D, Van Buren E, Jiang MZ, Chan CSY, Ye CJ, Perera RM, Gan L, Li Y, Shen Y. Functional characterization of Alzheimer’s disease genetic variants in microglia. Nature Genetics. 2023 Sep 21. doi: 10.1038/s41588-023-01506-8. PMID: 37735198
Cell line #4: WTC11-iNgn2-nCas9/RT
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iPSC line with stable expression of nCas9/RT and doxycycline inducible expression of Ngn2 (CLYBL locus knock-in of CAG-nCas9/RT with mCherry+Neomycin)
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Yang X, Jones IR, Chen PB, Yang H, Ren X, Zheng L, Li B, Li YE, Sun Q, Wen J, Beaman C, Cui X, Li Y, Wang W, Hu M, Ren B, Shen Y. Functional characterization of gene regulatory elements and neuropsychiatric disease-associated risk loci in iPSCs and iPSC-derived neurons. bioRxiv. 2023 Aug 30. doi: 10.1101/2023.08.30.555359.
Cell line #5: MCF7-nCas9/RT
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Human breast cancer cell line with stable expression of nCas9/RT (Lentivirus mediated random insertion of EF1a-nCas9/RT with Puromycin)
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Ren X, Yang H, Nierenberg JL, Sun Y, Chen J, Beaman C, Pham T, Nobuhara M, Takagi MA, Narayan V, Li Y, Ziv E, Shen Y. High throughput PRIME editing screens identify functional DNA variants in the human genome. Molecular Cell. 2023 Dec 21:2023.12.21; 83(24):4633-4645. doi: 10.1016/j.molcel.2023.11.021.